FastQCFastQC Report
Sun 19 Mar 2023
SRR4895982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697189
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCCA2712650.0143.17721
CTCCAAT947300.0142.60153
CCTCCAA2725250.0142.515232
CTCCAAG884450.0141.57413
TCCAATG321400.0141.266954
TCCAATA282550.0140.4074
TCCAAGT327200.0139.621034
CCAATAG94300.0137.815485
CTCCAAA730900.0137.754553
TCCAAGA251000.0136.857384
CCAATGT121300.0135.749375
CCAATGA89300.0134.566645
CCAATAT105450.0134.440965
TCCAAAT278050.0134.31544
TCCAATT350650.0133.959214
TCCAACG64950.0132.914544
CCAAGAT90250.0132.671465
CAATATG38750.0132.665826
CCAAGTA99750.0132.234595
CTCCAAC207900.0131.809523
Produced by FastQC (version 0.11.5)