FastQCFastQC Report
Sun 19 Mar 2023
SRR4895982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697189
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG193400.7170428175407804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT176790.6554601846589171No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC146620.5436029881480312No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA94590.35069844938563816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG63650.2359864288338711No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA54910.2035823221880261No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA47620.17655418289189226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA40520.15023048069675504No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC39030.14470621079946566No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG38690.14344563914505065No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC33430.12394385413851236No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC29810.11052247358268183No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT27720.10277366547171889No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC27360.10143894254351475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT691250.0135.58091
GTGGTAT692350.0135.251886
GGTATCA694150.0134.932288
TGGTATC695850.0134.633687
GTATCAA697350.0134.336249
CAGTGGT706650.0132.616424
AGCAGTG707250.0132.53412
AGTGGTA711400.0131.670565
GCAGTGG717650.0130.563283
TCAACGC693000.027.55745510-14
GTACATG694750.027.40255420-24
AGAGTAC695800.027.38820615-19
ATCAACG694850.027.38253810-14
CAGAGTA696400.027.37701615-19
CAACGCA698050.027.37459610-14
TACATGG696850.027.29724320-24
AGTACAT699450.027.13195220-24
CGCAGAG704200.027.00220715-19
GAGTACA695200.026.91669820-24
GCAGAGT710100.026.84477215-19
Produced by FastQC (version 0.11.5)