FastQCFastQC Report
Sun 19 Mar 2023
SRR4895981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334625
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCAG1340250.0143.392961
CAGCAGA1340650.0143.350172
AGCAGAT457450.0143.260253
GCAGATA144950.0142.460164
GCAGAGT143300.0141.990234
GCAGATG146950.0141.305194
AGCAGAC96900.0141.176473
GCAGATT159700.0140.618674
CAGATAG51100.0140.61845
GCAGAAT135200.0140.00594
AGCAGAA367000.0139.625063
CAGATGT50650.0138.598225
CAGAGTA43350.0138.352945
CAGATTG57150.0138.33075
GCAGAGA133750.0138.239994
CAGAGAT48250.0137.732645
CAGATGA45350.0137.64945
CAGATGG49500.0136.872715
GCAGACT32350.0136.432754
CAGAAGT41900.0135.408115
Produced by FastQC (version 0.11.5)