FastQCFastQC Report
Sun 19 Mar 2023
SRR4895981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334625
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT46190.34608972557834594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42750.320314695139084No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG40930.3066779057787768No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC31150.23339889482064252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC24250.18169897911398333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21280.1594455371358996No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC20410.15292685211201648No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA20120.15075395710405545No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG18340.13741687740001873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG17330.1298492085791889No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC16530.12385501545377915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC15420.11553807249227312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG15240.11418937903905592No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC14810.11096750023414817No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT14540.10894446005432239No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA14450.10827011332771377No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA13530.10137679123349255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT311550.0135.677766
TGGTATC312150.0135.416987
AAGCAGT312450.0135.361241
GGTATCA312400.0135.170328
GTATCAA313350.0134.691599
AGCAGTG316900.0133.490392
AGTGGTA318100.0132.906665
CAGTGGT319100.0132.535284
GCAGTGG322450.0131.148093
TCGTACG255.3706655E-428.79946150-54
TCAACGC311350.027.74497610-14
CAACGCA312250.027.67884310-14
GTACATG312000.027.64563620-24
AGAGTAC312050.027.61351815-19
ATCAACG311900.027.58986510-14
CAGAGTA312700.027.5561215-19
TACATGG313250.027.5307220-24
AGTACAT314650.027.3121220-24
CGCAGAG315600.027.23903515-19
CGACCTA8250.027.22858245-49
Produced by FastQC (version 0.11.5)