FastQCFastQC Report
Sun 19 Mar 2023
SRR4895980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688409
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGA1698050.0143.181661
CACAGAA1698700.0143.126882
ACAGAAT574700.0142.646943
ACAGAAG567600.0141.945023
CAGAATG191650.0141.595614
CAGAAGT201650.0140.893634
CAGAATA176200.0139.62774
CAGAATT204550.0138.825714
CAGAAAT168750.0138.410664
ACAGAAC116800.0135.924653
CAGAAGG202000.0135.481194
CAGAAAG169450.0135.077014
ACAGAAA480950.0134.538713
CAGAAGA171400.0133.708284
CAGAACG38550.0131.673164
CAGAACT41050.0129.617544
AGAATAG68250.0129.019795
AGAAGTG72050.0128.01115
AGAATTG78000.0127.661535
AGAATGG72550.0127.3273545
Produced by FastQC (version 0.11.5)