FastQCFastQC Report
Sun 19 Mar 2023
SRR4895980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688409
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT67290.3985408748709584No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59010.3495006245524633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39330.23294118901285174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC31390.1859146687798987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCACGCCTGTAATCCCT28840.1708116931383332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCACAGTGGCTCGTG28330.16779109801002007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA28090.16636964147904923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCGGGCGCGGTGGCTCA26840.15896622204690924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG22350.1323731394466625No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGGTGTGGTAGCTCAC20630.12218603430803791No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC19880.11774398264875394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC19830.11744784587146835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCACAGTGGCTCA17440.10329250791721674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG17420.1031740532063025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC17290.10240409758535994No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC17140.10151568725350316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT403600.0136.701816
GGTATCA403550.0136.683078
GTATCAA404550.0136.384849
TGGTATC404950.0136.263857
AAGCAGT406100.0135.872831
CAGTGGT410500.0134.513244
AGCAGTG412450.0133.842362
AGTGGTA412900.0133.692545
GCAGTGG426700.0129.440083
TAACGCG750.028.799608120-124
TCAACGC402150.027.96613310-14
ATCAACG403750.027.75544410-14
CAACGCA405550.027.75297710-14
GTACATG403550.027.75053420-24
TACATGG403550.027.75053420-24
AGAGTAC404400.027.72797815-19
CAGAGTA405750.027.63572315-19
AGTACAT405900.027.48344220-24
CGCAGAG410150.027.28307715-19
GAGTACA403500.027.24364720-24
Produced by FastQC (version 0.11.5)