FastQCFastQC Report
Sun 19 Mar 2023
SRR4895979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496219
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACA1500100.0143.625632
AAGGTAC1500300.0143.606481
GGTACAG483100.0142.673553
GGTACAT510450.0142.645893
GTACAGT178550.0141.983754
GTACAAG134200.0140.888234
GGTACAA408850.0140.742083
GGTACAC115000.0140.619133
GTACATA155500.0140.481024
GTACATG164200.0140.404394
GTACAAT154300.0140.313674
GTACAGA139100.0140.11794
GTACATT188750.0139.422524
GTACAGG161850.0139.151064
GTACACG36850.0138.333794
GTACACT39450.0138.15974
TACATAG56350.0137.611345
TACAGTA55700.0137.407555
TACAGTG61500.0135.336585
TACAGAT51900.0134.566485
Produced by FastQC (version 0.11.5)