FastQCFastQC Report
Sun 19 Mar 2023
SRR4895979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496219
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG67720.4526075394043252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC54270.3627142817996563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41750.27903669182118396No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCAGTGGCTCAC34460.2303138778480958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC31460.21026333711842987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC27620.1845986449844575No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG26940.18005385575239988No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC25270.16889238807955254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA23930.1599364798869684No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGGCACGGTGGCTC21780.1455669256973745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGTGAGTGGCTCAC21780.1455669256973745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20840.13928442293541254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA20630.1378808850843359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC19300.12899181202751736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTCCGGCGGTGACGACC17960.12003590383493326No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG16770.11208252267883244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT16690.11154784159270802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT374600.0136.040376
GGTATCA376200.0135.634038
AAGCAGT376450.0135.110671
TGGTATC378600.0134.736197
GTATCAA379850.0134.46349
AGCAGTG383950.0132.707752
CAGTGGT384850.0132.421544
AGTGGTA387350.0131.506715
GCAGTGG397350.0128.268623
CAACGCA373150.027.89650710-14
TCAACGC373900.027.82514210-14
TACGCAC10950.027.61596550-54
ATCAACG376500.027.56414810-14
GTACATG375500.027.53401420-24
AGAGTAC376300.027.51757415-19
TACATGG376300.027.51374620-24
CAGAGTA377500.027.43772715-19
CGCAGAG378650.027.31636815-19
AGTACAT377600.027.31224420-24
GAGTACA375150.027.10293420-24
Produced by FastQC (version 0.11.5)