FastQCFastQC Report
Sun 19 Mar 2023
SRR4895978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639597
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGA1645900.0143.444232
GACGGAT541600.0143.441443
AAGACGG1655550.0142.60811
ACGGATG177250.0142.496834
ACGGATA167200.0142.49264
ACGGAAT166550.0142.270584
GACGGAA456600.0142.202163
GACGGAG535950.0142.092163
GACGGAC122200.0141.760853
ACGGAGT184450.0141.696734
CGGATAG57500.0141.495455
ACGGATT190350.0141.314224
CGGATGA51050.0141.179025
CGGAGTA56700.0140.571215
ACGGACT42050.0140.232834
CGGATAT55750.0140.125355
CGGATTG66150.0139.863745
ACGGAGA154850.0139.72214
CGGAATA49200.0139.024195
CGGATTA53500.0138.75125
Produced by FastQC (version 0.11.5)