FastQCFastQC Report
Sun 19 Mar 2023
SRR4895978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639597
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG123200.7514041560212662No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC84070.5127479496486027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT72370.4413889510654142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51600.3147114809309849No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC27930.1703467376434575No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG26940.1643086685325723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC19740.12039543863522562No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG19390.11826076773743793No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG19330.11789482415496004No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA17320.10563571414195073No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16770.10228123130257008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA426500.0136.62088
GTGGTAT426400.0136.568426
TGGTATC428250.0136.146587
AAGCAGT428950.0135.695141
GTATCAA430000.0135.496159
CAGTGGT435750.0133.724734
AGCAGTG437100.0133.336322
AGTGGTA437200.0133.277165
GCAGTGG450850.0129.349693
CGTGGTA1656.3993866E-834.9085965
TCAACGC425700.027.92096310-14
CAACGCA427950.027.8111810-14
ATCAACG427100.027.74852210-14
AGAGTAC427600.027.73223715-19
TACATGG427950.027.61853620-24
CAGAGTA429800.027.61708615-19
GTACATG428600.027.60688620-24
AGTACAT431300.027.37730820-24
CGCAGAG433300.027.36077515-19
GAGTACA428100.027.1783120-24
Produced by FastQC (version 0.11.5)