FastQCFastQC Report
Sun 19 Mar 2023
SRR4895976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470060
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGA1472600.0143.749832
CCGAGAT477600.0143.712753
AACCGAG1474500.0143.564591
CGAGATA150600.0143.473284
CGAGAGT157700.0143.314334
CGAGATG162650.0142.759724
CCGAGAA417300.0142.394593
CGAGATT158450.0142.317924
CCGAGAG481500.0142.130043
CGAGAAT146200.0141.73384
CGAGAAG143050.0141.130284
CGAGAGA145000.0141.069534
CCGAGAC109050.0139.311463
CGAGACT33700.0138.871614
CGAGAGG170750.0137.294684
CGAGACG38450.0136.321734
CGAGAAA128100.0136.074174
CGAGATC9300.0135.48314
CGAGACA28600.0133.174074
GAGATAG60700.0125.376565
Produced by FastQC (version 0.11.5)