FastQCFastQC Report
Sun 19 Mar 2023
SRR4895976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470060
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG118520.8062255962341672No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC88890.6046691971756255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA40510.27556698366053084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36740.24992177190046663No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT35680.24271118185652285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG17330.1178863447750432No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA17220.11713807599689809No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGAGAGAGGCACTTTGC17220.11713807599689809No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC16950.11530141626872373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC15100.10271689590901052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA324150.0135.379538
AAGCAGT324200.0135.291631
TGGTATC324800.0135.219447
GTGGTAT324700.0135.216746
GTATCAA326100.0134.45969
CAGTGGT332100.0132.268814
AGCAGTG333050.0131.805852
AGTGGTA336300.0130.616935
GCAGTGG339000.0129.513693
TCAACGC322800.027.68876310-14
CAACGCA323800.027.62993210-14
AGAGTAC323600.027.5936115-19
CAGAGTA324000.027.56398815-19
GTACATG323350.027.55705320-24
ATCAACG323400.027.54388610-14
TACATGG326300.027.32556720-24
AGTACAT325450.027.3040220-24
CGCAGAG327350.027.21592515-19
GAGTACA324050.027.0220520-24
TATCAAC328200.026.7768910-14
Produced by FastQC (version 0.11.5)