FastQCFastQC Report
Sun 19 Mar 2023
SRR4895975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474826
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT24800.1681554298608785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACCA1498100.0141.75982
CAACCAT504600.0141.716453
CAACCAG496450.0141.47593
AACAACC1505550.0141.058321
AACCAGT175600.0139.325184
AACCATG184450.0139.120074
ACCATGA49700.0137.480335
ACCATAG51500.0137.288765
AACCATA146250.0137.254844
ACCAGTA56150.0136.81875
ACCATGT73050.0135.621635
CAACCAA411700.0134.660633
ACCATAT49500.0134.544925
ACCATTG67350.0134.164285
ACCAGTT61800.0133.16455
AACCAGG186600.0132.539664
AACCAGA146400.0132.491274
ACCATGG60300.0132.417395
ACCAGAT52650.0132.102045
AACCATT183600.0132.038684
Produced by FastQC (version 0.11.5)