FastQCFastQC Report
Sun 19 Mar 2023
SRR4895975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474826
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG135630.9196339093560867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG83440.5657616559512784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55000.372925348481787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC48450.3285133297080469No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG29500.20002359600386757No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT29340.19893872226282966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG27020.18320805301777973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21350.14476283981974822No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC20470.13879603424403963No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC16110.10923322480075616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA15480.10496153444541934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT599450.0128.042111
TGGTATC609400.0125.968747
GGTATCA609850.0125.863988
GTGGTAT609900.0125.806456
GTATCAA611300.0125.628589
AGCAGTG612100.0125.4536362
CAGTGGT613200.0125.176374
AGTGGTA615650.0124.619765
GCAGTGG623150.0123.240583
CATGGGG220450.026.39582825-29
ACATGGG596850.025.87549420-24
CAACGCA608800.025.78002410-14
AGAGTAC608200.025.70294815-19
TCAACGC610750.025.6882810-14
CAGAGTA609650.025.64417615-19
ATCAACG610500.025.60208910-14
GTACATG611050.025.57582320-24
TACATGG612600.025.51816420-24
GCAGAGT612600.025.4995315-19
AGTACAT611700.025.4591920-24
Produced by FastQC (version 0.11.5)