FastQCFastQC Report
Sun 19 Mar 2023
SRR4895974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236168
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG1239550.0143.604752
AGTACAA1240600.0143.48321
TACAAGT375900.0142.658943
TACAAGC138400.0141.866783
TACAAGA326400.0141.837953
ACAAGTG132600.0141.33914
ACAAGTA117800.0139.110084
ACAAGAT121050.0138.765524
CAAGTAG39850.0138.760075
ACAAGCT48200.0138.472764
CAAGATG42600.0136.732125
ACAAGTT127200.0136.697854
ACAAGCG48800.0136.475144
CAAGGTA45350.0135.267655
TACAAGG432600.0135.112083
CAAGTGT42500.0135.020925
CAAGTAT37900.0134.691025
CAAGGAT39050.0134.412035
ACAAGAG114450.0134.374574
CAAGATA35700.0134.319065
Produced by FastQC (version 0.11.5)