FastQCFastQC Report
Sun 19 Mar 2023
SRR4895974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236168
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT102470.8289326369878528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41600.3365238381838067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA37630.3044084622802079No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG32230.26072507943904066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC29400.23783175102413268No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG26280.21259246316034713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC25080.2028850447511989No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG23140.1871913849897425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG22290.18031529694992912No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC21120.17085056400100956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA20140.16292283896687182No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT18960.1533772108645427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC18710.15135483202930347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG16180.13088835821668252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC15300.12376958471664046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA14980.12118093980753425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC14920.12069556888707683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG14130.11430485176772089No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA13700.11082636017110943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA12560.10160431268241858No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC12550.10152341752900901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCACACCCGTCTATGTAG12420.10047178053468461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT369150.0136.133511
GTGGTAT368800.0136.111376
GGTATCA369600.0135.77788
TGGTATC369900.0135.66777
GTATCAA371700.0135.068829
AGCAGTG373750.0134.38282
AGTGGTA374350.0134.093445
CAGTGGT376450.0133.402794
GCAGTGG379950.0132.20893
CGTGGTA1352.2465823E-531.9996665
CAACGCA368800.027.74157710-14
TCAACGC368800.027.71815110-14
AGAGTAC369850.027.57716215-19
CAGAGTA370350.027.56326115-19
GTACATG370300.027.528120-24
ATCAACG370350.027.49716210-14
TACATGG371900.027.40966420-24
CGCAGAG372000.027.35584415-19
AGTACAT372700.027.27741820-24
GCAGAGT375200.027.18393915-19
Produced by FastQC (version 0.11.5)