FastQCFastQC Report
Sun 19 Mar 2023
SRR4895973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948836
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT950100.0143.802982
AACGTGA950350.0143.765141
CGTGATG392900.0143.633483
CGTGATA213500.0143.42673
CGTGATT244800.0142.852943
CGTGATC102700.0142.177223
GTGATGA94850.0140.584094
GTGATCA27550.0140.34124
GTGATGT199400.0139.23374
GTGATCG22950.0138.666674
GTGATAA61900.0138.300484
GTGATGG101150.0137.522494
GTGATAG51500.0137.00974
GTGATAT105600.0136.977284
GTGATTA69600.0134.482764
GTGATCT47050.0134.35924
TGATGTG85200.0134.028175
TGATGAG39400.0133.76655
TGATATG43400.0133.548395
TGATCAG11100.0132.972985
Produced by FastQC (version 0.11.5)