FastQCFastQC Report
Sun 19 Mar 2023
SRR4895973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948836
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG43210.45540009021580125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC39080.4118730739558786No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35840.37772597161153243No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT20330.21426252798165332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG15890.16746835069495677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA12700.1338482098065419No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC12320.12984330274146427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA11660.12288741152317155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG11020.11614230488725132No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC10240.10792170617472355No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG10010.10549768347743972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCGGTGGCTCATG9960.10497072202150846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT225650.0137.386921
GTGGTAT226300.0136.839366
GGTATCA226800.0136.601178
TGGTATC227350.0136.27077
GTATCAA228350.0135.579369
CAGTGGT231450.0133.981194
AGCAGTG231400.0133.952092
AGTGGTA233750.0132.570475
GCAGTGG239000.0129.66243
TACGCAC3600.028.39958450-54
TCAACGC225900.027.97090310-14
AGAGTAC226300.027.92782815-19
CAACGCA227000.027.86708310-14
TACATGG226500.027.83959220-24
ATCAACG226350.027.83895510-14
GTACATG226450.027.83302120-24
CAGAGTA227200.027.81719815-19
AGTACAT228200.027.56909620-24
CGCAGAG229950.027.41564615-19
ACATGGG229850.027.3210720-24
Produced by FastQC (version 0.11.5)