FastQCFastQC Report
Sun 19 Mar 2023
SRR4895972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540501
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGC1543350.0143.734091
TGTAGCC1545500.0143.534132
GTAGCCG474300.0143.45353
GTAGCCT491100.0143.28163
GTAGCCA420900.0142.870993
TAGCCGT168850.0142.76344
TAGCCGA141350.0142.726564
TAGCCAT146550.0142.722634
TAGCCTA150250.0142.658234
TAGCCTG154300.0142.226844
TAGCCGG156950.0141.66044
TAGCCAG134250.0141.532964
AGCCGAT51300.0141.192985
GTAGCCC165850.0140.440163
AGCCGTA55150.0140.344535
TAGCCAA136350.0139.88124
TAGCCTT182600.0139.85984
TAGCCCG51500.0138.966984
AGCCTAT53000.0138.837745
AGCCGTG50050.0138.677325
Produced by FastQC (version 0.11.5)