FastQCFastQC Report
Sun 19 Mar 2023
SRR4895972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540501
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG146790.9528718254645728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC97610.6336250349723889No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55520.3604022327801151No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46680.30301830378558664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA34900.2265496744240997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC26880.17448868906933523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC24650.16001287892705035No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT22970.1491073358602169No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC21920.142291371443446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC20070.13028229128056393No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGAGAGAGGCACTTTGC18850.12236278976774438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG18390.11937674821373048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACTCTTAGCGGTGGATCA16610.10782206567863312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTCCGGCGGTGACGACC16270.10561499148653587No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG16050.1041868846563553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGACCTCAGATCAGACGT15540.10087627336820944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT469550.0132.890521
GTGGTAT471650.0132.36566
GGTATCA471850.0132.324748
TGGTATC476050.0131.202657
GTATCAA476550.0131.008839
AGCAGTG478600.0130.370092
CAGTGGT481150.0129.790474
AGTGGTA482850.0129.280365
GCAGTGG487350.0128.08853
CGTGGTA1453.6608668E-529.7926395
CAACGCA470950.027.04842610-14
CGACCTA9000.027.0395845-49
TCAACGC471000.027.01192310-14
GTACATG470850.026.91872820-24
TACATGG471900.026.88019220-24
ATCAACG472300.026.84305410-14
AGAGTAC472550.026.84034515-19
CAGAGTA473600.026.78691915-19
AGTACAT473600.026.67120620-24
CGCAGAG475200.026.63006215-19
Produced by FastQC (version 0.11.5)