FastQCFastQC Report
Sun 19 Mar 2023
SRR4895971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842186
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT103460.5616153851999743No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG88610.48100463253981957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC74250.40305376330077414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57550.3124005936425529No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37770.20502815676592917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA33950.1842919227483001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA30020.16295857204429953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG26600.14439367143165782No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC24360.13223420436372874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA24330.13207135435835468No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC22420.12170323734954018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG19890.10796955356299526No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC19780.10737243687662375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGGTAGTGACGAAAAA18990.10308405340177376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT554250.0137.483551
GTGGTAT557550.0136.688776
TGGTATC559200.0136.375587
GGTATCA559550.0136.328878
GTATCAA559850.0136.217259
CAGTGGT566650.0134.649774
AGCAGTG567450.0134.37842
AGTGGTA570200.0133.744695
GCAGTGG574200.0132.886523
CGTGGTA1352.2472545E-531.9995045
TCAACGC556950.027.90756610-14
CAACGCA559250.027.83656310-14
ATCAACG558300.027.77044710-14
GTACATG558450.027.73462320-24
AGAGTAC560900.027.66739315-19
CAGAGTA561550.027.64049715-19
TACATGG560750.027.62620-24
AGTACAT561550.027.52510520-24
CGCAGAG563950.027.48456815-19
CGACCTA7000.027.35987345-49
Produced by FastQC (version 0.11.5)