FastQCFastQC Report
Sun 19 Mar 2023
SRR4895970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962550
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC2975150.0141.97241
TCATTCC2979600.0141.760382
CATTCCG1019250.0141.604033
ATTCCGG346900.0139.966374
CATTCCA679850.0139.734563
CATTCCT887800.0139.35633
ATTCCGA256600.0139.297974
ATTCCGT339400.0139.047534
ATTCCTG297450.0137.135854
ATTCCTA242000.0136.71414
ATTCCAG223300.0135.520844
CATTCCC442450.0134.117363
TTCCGAT91200.0133.508885
ATTCCGC96250.0133.392094
TCCGATA24500.0132.974156
ATTCCAT237350.0132.934984
ATTCCCG115750.0132.783744
TTCCGTA95650.0132.365595
TTCCGAG87400.0132.217445
TTCCGGA90450.0131.620945
Produced by FastQC (version 0.11.5)