FastQCFastQC Report
Sun 19 Mar 2023
SRR4895970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962550
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA275210.9289632242493798No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC262490.8860272400465816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG148450.5010885892221228No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135500.4573762468143998No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGGTAGTGACGAAAAA127050.42885352145955347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC110770.3739008624327016No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA95390.321986126816425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA66470.2243675212232705No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCATCAATAAACACTGTTA65590.22139710722181905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCCCGACCCGGGGAGGTA60250.20337209498573866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGATCCATTGGAGGGCAA58990.19911900221093315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTTTTTCCAAATGATCCTA57160.1929418912760966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATGATCTTGTTACTGT53650.18109398997485276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA50750.1713051256518877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCACAAAAAAAAAAAAA47810.16138124251067493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA44760.1510860576192807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGATCTTGTTACTGTGATA43930.14828441714063897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTGTTTTAATAAAAGATT40820.13778670402187304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA37060.12509493510658048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG35350.11932288062648731No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCAATAAACACTGTTACAG33870.11432718435131896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG30270.10217549070901757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1782400.0141.418811
GGTATCA1810700.0139.390728
GTATCAA1813050.0139.233879
GTGGTAT1817450.0138.829456
TGGTATC1818850.0138.762167
AGCAGTG1817000.0138.670362
AGTGGTA1819950.0138.654575
CAGTGGT1822000.0138.542024
GCAGTGG1832050.0137.664893
ACGCAGT4400.031.102471
TCAACGC1808300.028.50141310-14
CAACGCA1808600.028.50066610-14
ATCAACG1807700.028.47423410-14
AGAGTAC1800250.028.45077715-19
GTACATG1797650.028.37055420-24
CAGAGTA1805600.028.37046415-19
TACATGG1795800.028.3604920-24
CGCAGAG1812350.028.35934815-19
AACGCAG1818750.028.34319710-14
AGTACAT1800550.028.31206320-24
Produced by FastQC (version 0.11.5)