FastQCFastQC Report
Sun 19 Mar 2023
SRR4895969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005398
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC1006050.0142.434222
ATAGCGA1006300.0142.398831
AGCGACT292150.0142.021133
AGCGACG320650.0141.799943
GCGACTA77250.0141.426334
AGCGACC154550.0141.242253
AGCGACA244500.0141.13433
GCGACTG99150.0140.88024
GCGACGT99500.0140.52794
GCGACTT86950.0139.828634
GCGACGA85800.0139.460134
CGACTAA21100.0139.162755
GCGACAT80200.0139.067814
GCGACAA64250.0138.651154
GCGACAG81200.0138.408344
CGACTGA25550.0138.356325
CGACGAT28350.0138.266785
CGACTAG28000.0138.213365
CGACGTA28000.0137.958835
CGACGTT32500.0137.716035
Produced by FastQC (version 0.11.5)