FastQCFastQC Report
Sun 19 Mar 2023
SRR4895969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005398
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG105161.0459539406284875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC77530.7711374003131098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC54340.5404824755967289No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30460.30296459710482815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC29740.2958032540347206No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT25010.24875720858804173No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA23080.22956083063622565No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG20220.2011143845521873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA14710.14631021744622527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA14280.14203330422379992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA13500.13427518256451673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT13210.13139075271683454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC12600.12532350372688228No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC10750.1069228305606337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATTCCCCCAGTAGCGGC10180.1012534339634652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT302350.0139.770461
GGTATCA303150.0139.455028
GTGGTAT304650.0138.721126
GTATCAA305050.0138.610029
TGGTATC307550.0137.459877
CAGTGGT308000.0137.352554
AGCAGTG309550.0136.49622
AGTGGTA310400.0136.174585
GCAGTGG311000.0135.882953
CAACGCA303000.028.57011610-14
ATCAACG303050.028.49888210-14
AGAGTAC302500.028.48405615-19
GTACATG302050.028.4423820-24
TCAACGC304300.028.42440210-14
CAGAGTA303750.028.38580115-19
AGTACAT302650.028.34317420-24
TACATGG303100.028.30584520-24
CGCAGAG304900.028.26456815-19
GCAGAGT306550.028.08895115-19
GAGTACA302100.027.81801420-24
Produced by FastQC (version 0.11.5)