FastQCFastQC Report
Sun 19 Mar 2023
SRR4895968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842459
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAA843700.0142.319661
GGCTAAC843850.0142.294362
GCTAACG273650.0142.062073
GCTAACT243800.0141.946213
GCTAACA199850.0141.568023
GCTAACC129650.0141.156653
CTAACGA67100.0141.150134
CTAACGG93400.0141.08114
CTAACTG84100.0141.005524
CTAACGT90200.0140.871674
CTAACTA62750.0138.783024
CTAACAT67000.0138.701664
TAACGAT25100.0138.555885
CTAACCG36650.0137.864064
TAACGTG31550.0137.787325
TAACGTA24600.0137.605995
CTAACTT76550.0136.945044
TAACGGA23500.0136.162435
TAACGTT29550.0135.536935
TAACGAG21750.0135.322375
Produced by FastQC (version 0.11.5)