FastQCFastQC Report
Sun 19 Mar 2023
SRR4895968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842459
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG110231.3084316269397087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC70340.8349367743712157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC51520.6115431136708136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23490.2788266253906718No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA22260.26422650835233524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21420.254255696716398No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT20960.24879549034433723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT20290.24084258106329212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC18930.2246993622241557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA17020.20202763576625096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC14710.1746079037674237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA12580.14932477426201157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA12400.14718817176859644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT271350.0141.057821
GGTATCA273950.0139.529628
GTATCAA275900.0138.517369
GTGGTAT276200.0138.21056
CAGTGGT277450.0137.691624
AGCAGTG278300.0137.457532
TGGTATC278500.0137.198357
AGTGGTA279400.0136.679115
GCAGTGG280950.0136.058443
CGCACGA304.6134097E-528.8012160-64
AGAGTAC271600.028.5732215-19
CAACGCA273700.028.54764410-14
CAGAGTA272550.028.50004415-19
GTACATG271100.028.48080620-24
ATCAACG274450.028.44339810-14
TCAACGC275600.028.3665110-14
TACATGG272350.028.29721620-24
AGTACAT273200.028.26715520-24
CGCAGAG275700.028.22142615-19
GCAGAGT276350.028.09772515-19
Produced by FastQC (version 0.11.5)