FastQCFastQC Report
Sun 19 Mar 2023
SRR4895967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301638
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93220.7161745431525509No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA49460.37998276018370697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC44720.34356710544713664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG38810.29816277644014694No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC29850.22932643330941474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA23040.17700773947902565No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA19640.15088680570173887No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA16430.12622557116494756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCAAAAAAAAAAAAAAA15250.11716007061871272No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAAAAAAAAAAAAAAA14130.1085555277273712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA13210.10148751035234066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA13090.10056559504255408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT982200.0141.929241
GGTATCA998850.0139.837698
AGCAGTG997800.0139.645312
GTATCAA1001500.0139.474859
GTGGTAT1000850.0139.392796
AGTGGTA1001350.0139.351945
TGGTATC1002100.0139.333887
CAGTGGT1003250.0139.15984
GCAGTGG1006600.0138.56763
CGGTATC1203.8526196E-947.9989247
AGCGGTA1307.8198354E-944.3066985
GCGGTAT1401.5044861E-841.1419376
ACGCAGT1854.4638E-935.037011
CAACGCA997750.028.6088510-14
ATCAACG998350.028.53973210-14
TCAACGC999400.028.53712310-14
AGAGTAC992050.028.49453715-19
AACGCAG1000700.028.49429510-14
CGCAGAG999250.028.46214715-19
CAGAGTA993800.028.45450415-19
Produced by FastQC (version 0.11.5)