FastQCFastQC Report
Sun 19 Mar 2023
SRR4895966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350148
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG179301.3280025597193788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC139251.0313684129443585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC84630.626820170825717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG62620.4638010055193949No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37430.27722886676127356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC32720.24234380230908018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA23780.1761288392087386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT23750.17590664134598577No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCAGTGGCTCAC20620.15272399766544112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA18570.13754047704399813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCTTGCTGCAGCAACG15640.11583915244847232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA350000.0139.554928
AAGCAGT351900.0138.770871
GTGGTAT352200.0138.662756
TGGTATC354350.0137.923027
GTATCAA355400.0137.515539
CAGTGGT360250.0135.664184
AGCAGTG360350.0135.436842
AGTGGTA361450.0135.154045
GCAGTGG365450.0133.586183
CAACGCA351150.028.4228110-14
ATCAACG351050.028.41039710-14
TCAACGC352000.028.3664510-14
AGAGTAC350450.028.35694315-19
GTACATG349400.028.31746720-24
TACATGG349200.028.2594620-24
CAGAGTA352500.028.17977515-19
AGTACAT351000.028.17607720-24
CGCAGAG356500.027.91610115-19
GAGTACA349350.027.77330820-24
TATCAAC356100.027.47371310-14
Produced by FastQC (version 0.11.5)