FastQCFastQC Report
Sun 19 Mar 2023
SRR4895965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256782
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC1258800.0142.300812
AGATGTA1260400.0142.120161
ATGTACG436450.0142.05943
ATGTACC194600.0140.884933
TGTACGA102100.0140.78794
TGTACGG159100.0140.203664
TGTACGT140650.0140.202134
ATGTACT353650.0139.95353
ATGTACA287950.0139.661973
GTACGTA34700.0139.657625
TGTACGC40250.0139.16884
TGTACTA84300.0138.728674
TGTACCG54150.0138.321434
GTACGGT49600.0138.078775
GTACGTG46550.0137.633835
GTACGAG36450.0137.449455
TGTACTG121950.0137.214974
GTACGAT33400.0136.558495
GTACGTT45300.0136.240075
TACGTAG14350.0135.58016
Produced by FastQC (version 0.11.5)