FastQCFastQC Report
Sun 19 Mar 2023
SRR4895965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256782
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG61390.4884697584783996No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC47360.37683544162790367No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC44310.3525671118777958No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37030.29464139365458764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG32910.2618592564183765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC32210.25628947581999106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30980.24650257562568528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC23230.18483714757213263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG13410.10670108260621174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCTCGGTGGCTCA13310.10590539966358525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA13180.10487101183817082No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC12730.10129043859635163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT350900.0135.341221
GGTATCA352250.0135.045338
GTGGTAT354650.0134.131446
TGGTATC356750.0133.32177
GTATCAA358400.0132.788279
AGTGGTA359550.0132.32355
CAGTGGT359750.0132.309974
AGCAGTG360400.0131.75372
GCAGTGG364500.0130.350723
CAACGCA351200.027.6970810-14
TCAACGC352600.027.55443810-14
TACATGG351600.027.5185720-24
GTACATG352400.027.46835720-24
ATCAACG353600.027.41542610-14
AGAGTAC355000.027.34447115-19
CAGAGTA356900.027.198915-19
CGCAGAG356400.027.18048915-19
AACGCAG359250.027.10851910-14
AGTACAT358750.026.97011820-24
GAGTACA353250.026.85193320-24
Produced by FastQC (version 0.11.5)