FastQCFastQC Report
Sun 19 Mar 2023
SRR4895964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271918
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC1275400.0142.153172
AGATTCG425150.0142.091723
ACAGATT1277500.0141.91951
GATTCGA106550.0141.340324
AGATTCA301050.0141.171223
GATTCGG141450.0140.680044
AGATTCT386050.0140.310443
GATTCGT146150.0140.203554
AGATTCC173650.0139.507133
GATTCTA103200.0138.538744
GATTCGC35900.0138.176684
GATTCAT100400.0138.001114
GATTCTG135600.0137.708764
GATTCAA84100.0137.544274
ATTCGAG35250.0137.287385
ATTCGAA29500.0137.229135
GATTCCG44350.0137.080414
ATTCGTA39550.0136.95795
ATTCGAT37250.0136.038975
ATTCGGT50750.0135.382035
Produced by FastQC (version 0.11.5)