FastQCFastQC Report
Sun 19 Mar 2023
SRR4895963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665180
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC1670150.0142.14671
ACGACCT455600.0141.9323
CACGACC1672700.0141.932
ACGACCG554550.0141.815083
CGACCGT170350.0141.192574
ACGACCA387450.0141.0283
CGACCAT120200.0140.972234
CGACCTA125750.0140.816154
GACCGTA48250.0140.061875
CGACCTG155650.0139.917144
CGACCGA143050.0139.683174
GACCTAT43450.0139.128135
ACGACCC284050.0138.909413
CGACCGG191550.0138.814744
CGACCTT142950.0138.234974
GACCGAT47500.0138.221275
CGACCAG132900.0137.960534
GACCGTG53950.0137.684545
GACCTGT52150.0136.148825
GACCGTT58600.0136.004445
Produced by FastQC (version 0.11.5)