FastQCFastQC Report
Sun 19 Mar 2023
SRR4895963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665180
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA194621.1687625361822747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG134780.8094019865720222No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC97180.5836005717099653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA60890.3656661742274108No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57500.3453080147491563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA57400.3447074790713316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC46050.2765466796382373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG33810.20304111267250385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT22930.13770283092518526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAT22190.1332588669092831No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC21410.12857468862225105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19360.11626370722684636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATCCCAAGGCCCAACTC17200.10329213658583455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT402750.0139.828921
GGTATCA405950.0138.87188
GTGGTAT406750.0138.545556
GTATCAA409300.0137.805519
TGGTATC411300.0137.10047
AGCAGTG412350.0136.521122
CAGTGGT413050.0136.502124
AGTGGTA415750.0135.563695
GCAGTGG419200.0134.393373
AGAGTAC401750.028.48851415-19
ATCAACG405250.028.48003410-14
TCAACGC405950.028.45930510-14
CAACGCA406500.028.43496710-14
CAGAGTA403150.028.39315815-19
GTACATG401500.028.37628220-24
TACATGG401300.028.336620-24
AGTACAT403450.028.25697720-24
CGCAGAG410450.028.11621915-19
GCAGAGT408950.027.99750715-19
GAGTACA402050.027.76799620-24
Produced by FastQC (version 0.11.5)