FastQCFastQC Report
Sun 19 Mar 2023
SRR4895962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981330
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC984850.0142.021032
AATGTTG988700.0141.467991
TGTTGCG332500.0140.369983
GTTGCGA80350.0139.962624
TGTTGCA223600.0139.666263
GTTGCAT73550.0138.271364
GTTGCTA73550.0138.077584
GTTGCAG75250.0137.704734
TGTTGCT285300.0137.688723
GTTGCGT105150.0137.654864
GTTGCGG118550.0137.004154
TTGCGTA28100.0136.701525
GTTGCTG99000.0135.120124
TGTTGCC168600.0134.545753
TTGCGAG26650.0134.512195
TTGCGAT28900.0134.15045
GTTGCAA60050.0133.871144
TTGCGGA29650.0133.401035
TTGCTAG28000.0132.608285
GTTGCCG45200.0131.497824
Produced by FastQC (version 0.11.5)