FastQCFastQC Report
Sun 19 Mar 2023
SRR4895962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981330
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG107831.0988148736918264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC73950.7535691357647274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC50820.5178686068906484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC41840.42636014388635834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG35400.3607349209745957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28400.2894031569400711No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC26700.27207972853168655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT23480.2392671170758053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA22530.2295863776711198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG16760.1707886236026617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT14590.1486757767519591No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC14340.14612821375072604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA11650.11871643585745874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC11410.11627077537627506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCTTGCTGCAGCAACG11250.11464033505548593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC11140.1135194073349434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCCCCTCATTTACATAAA10170.10363486289015927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT314000.0138.267091
GGTATCA317050.0137.090738
GTGGTAT317150.0136.91136
TGGTATC318600.0136.378597
GTATCAA320650.0135.59659
CAGTGGT320600.0135.505374
AGCAGTG323050.0134.28222
AGTGGTA323950.0134.081865
GCAGTGG324750.0133.667953
CAACGCA316400.028.11616710-14
TCAACGC316300.028.10229110-14
ATCAACG317000.028.0084410-14
TACATGG314350.027.97486320-24
GTACATG314450.027.96596720-24
AGAGTAC316050.027.94683515-19
CAGAGTA316100.027.9378615-19
AGTACAT316100.027.7789920-24
CGCAGAG318500.027.76350815-19
GCAGAGT320550.027.51407815-19
AACGCAG323800.027.50918610-14
Produced by FastQC (version 0.11.5)