FastQCFastQC Report
Sun 19 Mar 2023
SRR4895961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126210
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC68305.411615561365977No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA23341.8492987877347278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA21931.7375802234371285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21681.7177719673559941No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA20901.655970208382854No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA15901.2598050867601618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATTCCCCCAGTAGCGGC13071.0355756279217179No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGAAATCAACCGAGAT11210.8882022026780761No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAGCAAAAAAAAAAAAAAA10280.8145154900562555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGCAGCCC10160.8050075271373108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGAGATTCCCCCAGTAG9660.7653910149750416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAATCAACCGAGATTCCCC8310.6584264321369147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATTCCAAAAAAAAAAAAA7730.6124712780286824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTAATGAGGCGAACC7330.5807780682988669No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC7320.5799857380556216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7290.5776087473258854No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGACTAAGCGTACACGGT7280.5768164170826401No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA7130.5649314634339593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAACCGAGATTCCCCCAGTA7080.5609698122177323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGATTCCCCCAGTAGCGG6070.4809444576499485No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGAGATTCCCCCAGTAGCG6070.4809444576499485No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACCGGGGGAACTGAAACA5650.4476665874336423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCAAAAAAAAAAAAAAA5620.44528959670390617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGAAAAAAAAAAAAAAAA5530.4381586245146977No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTAAGTACCCCGAGGAAA5400.4278583313525077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4720.3739798748118216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCATCTAAGTACCCCGAGGAA4570.3620949211631408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTACAGCAAAAAAAAAAAA4460.35337928848744155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCATCTCCTCTAACT4410.34941763727121466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCATCTCCTCTAAC4240.3359480231360431No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA4180.3311940416765708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGACTAAGCGTACACGG4080.3232707392441169No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTC3730.2955391807305285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCT3320.26305364075746773No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGGACGTGCTAATCTGCG3230.2559226685682593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTAAGCGTACACGGTGGA3080.24403771491957849No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA3050.24166072418984236No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCAGAGCCTGAATCAGT3020.23928373346010617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGAACGGGGAGCAGCCCA2990.23690674273037002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAAAAAAAAAAAAAAA2940.23294509151414308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAAAAAAAAAAAAAAAAA2940.23294509151414308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTGTGAGGTTAAGCGACTA2880.2281911100546708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATTCCCCCAGTAGCGGCG2840.22502178908168927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCCACAAAAAAAAAAAAA2830.22422945883844386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAGCGTACACGGTGGATG2780.22026780762221693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2660.21075984470327233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGAAGGACGTGCTAATCT2470.19570557008161002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCGTCGGTAAGGTGATATG2370.18778226764915618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA2240.17748197448696615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCGTACACGGTGGATGCC2210.17510498375723002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACGGGGAGCAGCCCAGAG2210.17510498375723002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGACTAAGCGTACACGGTG2150.1703510022977577No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCGTACACGGTGGATGC2110.16718168132477618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGACTAAGCGTACACGGTGG2060.16322003010854924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA2060.16322003010854924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGGCAGTCAGAGGCGATGA2020.1600507091355677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAATTCTGGACATTAAT2010.15925837889232233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCTGGCAGTCAGAGGCG1930.15291973694635924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATTCCCCCAGTAGCGGCGA1750.13865779256794233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1690.13390381110847002No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACGTGCTAATCTGCGATA1630.12914982964899768No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAGTACCCCGAGGAAAAG1620.1283574994057523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCAAAAAAAAAAAAAAAA1610.12756516916250693No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGCAGCCCAGAGCCTG1590.12598050867601618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGGGGGAACTGAAACAT1530.12122652721654385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGAAAAAAAAAAAAAAAA1480.11726487600031692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTTAAGCGACTAAGCGTA1470.11647254575707154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGCGAACGGGGAGCAGCC1460.11568021551382615No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACA1440.1140955550273354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA1430.11330322478409001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGGAGCCC1390.11013390381110846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGTACCCCGAGGAAAAGA1310.10379526186514539No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGAAAAAAAAAAAAAAAAA1300.1030029316219No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGCTTGCAATCCCAATAA1280.10141827113540923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGCGGCGAGCGAACGGGG1280.10141827113540923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTA100.0072326693143.993265
AAGCAGT85550.0143.29261
CAGTGGT87700.0140.134844
AGTGGTA87650.0140.132635
AGCAGTG87400.0140.012312
GTATCAA88200.0139.666959
GCAGTGG87900.0139.543623
GGTATCA88200.0139.50378
GTGGTAT89750.0136.853766
TGGTATC90600.0135.569827
GGCAGTG353.539102E-582.3144762
AGGCAGT451.2291566E-464.022381
GTGGTAA750.028.81006680-84
GTAACTT200.006270877628.81006635-39
TAACGGT800.028.80321985-89
GACTGAA800.028.80321930-34
ATGGGTG900.028.79865525-29
GGGGATA453.0107913E-828.79865525-29
TGGGGTC552.3101165E-1028.79865525-29
GGGCCGA552.3101165E-1028.79865525-29
Produced by FastQC (version 0.11.5)