FastQCFastQC Report
Sun 19 Mar 2023
SRR4895960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075426
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACAC1081650.0141.717992
GGACACT317100.0141.35043
AAGGACA1085000.0141.280411
GACACTA86400.0140.891824
GACACGT107750.0140.887994
GGACACG335350.0140.75643
GACACTG99150.0140.169514
GACACGA85550.0139.54254
GGACACA274400.0139.476763
GACACAT92800.0139.085594
GACACTT104550.0139.064994
GACACGG114250.0137.363954
GGACACC168350.0136.572393
GACACTC31150.0135.220054
ACACGTA32850.0134.948075
GACACAG91750.0134.6964
ACACGAT32100.0133.660525
ACACGGT39200.0133.269135
ACACTGT38700.0132.596855
ACACTAG30350.0132.20915
Produced by FastQC (version 0.11.5)