FastQCFastQC Report
Sun 19 Mar 2023
SRR4895960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075426
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG102900.956830130571513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC76910.7151584581365896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC41770.3884042230706715No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA40090.37278250665317747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC35270.3279630583601289No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34770.3233137379977795No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT29240.27189225479019474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21910.2037332182781521No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTAAGCTGCAGCAAGA18490.171931866999682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG16570.15407847680826017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA15070.14013051572121188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG14290.13287757595594676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA13890.12915811966606722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC12550.11669794109497073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT284100.0139.567031
GGTATCA286250.0138.738088
GTATCAA287500.0138.134879
GTGGTAT287500.0137.959586
TGGTATC290250.0136.80137
CAGTGGT291000.0136.49824
AGCAGTG290700.0136.348792
AGTGGTA292250.0135.79125
GCAGTGG295150.0134.585833
CAACGCA286000.028.41632510-14
ATCAACG285500.028.37530510-14
TACATGG282650.028.335920-24
AGAGTAC285150.028.31923915-19
TCAACGC286950.028.29715710-14
GTACATG283850.028.27698120-24
CAGAGTA285700.028.26975815-19
AGTACAT284950.028.15266420-24
CGCAGAG288500.028.0702615-19
GCAGAGT288600.027.94078815-19
TATCAAC286900.027.67973310-14
Produced by FastQC (version 0.11.5)