FastQCFastQC Report
Sun 19 Mar 2023
SRR4895959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450251
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGATC1453750.0142.20512
AAGAGAT1458100.0141.780851
GAGATCG434100.0141.727343
GAGATCA361950.0141.445543
GAGATCT467900.0141.329653
AGATCGT152450.0140.818444
AGATCGA115200.0140.754064
GAGATCC198500.0140.465713
AGATCTA123500.0139.835824
AGATCAG111850.0139.489584
AGATCAT127750.0139.033394
GATCGTA44500.0139.024635
AGATCTG149050.0139.009694
AGATCCG49350.0138.793214
GATCGAT41750.0138.45115
AGATCGC44000.0138.336624
GATCGAG38000.0138.046775
AGATCAA100800.0137.315864
AGATCGG129600.0136.993744
GATCTAG39700.0136.982795
Produced by FastQC (version 0.11.5)