FastQCFastQC Report
Sun 19 Mar 2023
SRR4895959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450251
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG187691.2941897643925087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC137110.9454225509928971No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC72300.49853439163289665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50700.3495946563732761No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC29280.20189608557415234No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC22150.1527321822222498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA20760.14314763444396866No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA19690.13576960126212634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT19480.13432157605821338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG18710.12901215031053245No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC17880.12328900307601925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT17200.11860015955858676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCACACCTGTAATCCCA14920.10287874305896015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA419800.0139.449058
AAGCAGT419750.0139.106751
GTATCAA422100.0138.689189
GTGGTAT423700.0138.046526
TGGTATC425100.0137.659627
CAGTGGT427350.0136.901154
AGCAGTG427950.0136.508642
AGTGGTA431450.0135.550145
GCAGTGG431950.0135.277853
CAACGCA419150.028.49989310-14
AGAGTAC417250.028.4847315-19
TCAACGC419800.028.46262710-14
CAGAGTA418550.028.42377915-19
ATCAACG420450.028.39465110-14
GTACATG418200.028.35272220-24
TACATGG419050.028.26428420-24
CGCAGAG421550.028.23174715-19
AGTACAT420200.028.19721420-24
GCAGAGT422450.028.1272915-19
GAGTACA418350.027.80558420-24
Produced by FastQC (version 0.11.5)