FastQCFastQC Report
Sun 19 Mar 2023
SRR4895958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053963
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG134751.2785078793088562No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC92310.8758371973209685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC64340.6104578623727778No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA33390.316804290093675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30880.2929894123417995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC21020.19943774117307722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT18020.17097374386007858No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA17200.1631935845945256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG14940.1417507066187333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA13150.12476718822197744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT13090.12419790827571746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA12470.11831534883103108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG12430.11793582886685774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC11790.11186350944008472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA11480.10892222971774151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT273700.0139.217671
GGTATCA274350.0138.933618
GTATCAA276200.0137.95099
GTGGTAT276900.0137.680166
TGGTATC276900.0137.654167
CAGTGGT279650.0136.351994
AGCAGTG280750.0135.773032
AGTGGTA281250.0135.576315
GCAGTGG285300.0133.531983
CAACGCA274150.028.45884910-14
AGAGTAC273600.028.36949715-19
ATCAACG274600.028.35452710-14
TCAACGC275300.028.33473810-14
GTACATG273700.028.25782420-24
TACATGG273150.028.2514620-24
CAGAGTA275350.028.17873415-19
CGCAGAG277300.028.06366715-19
AGTACAT275300.028.04651820-24
GCAGAGT277850.027.91482415-19
AACGCAG282300.027.70355410-14
Produced by FastQC (version 0.11.5)