FastQCFastQC Report
Sun 19 Mar 2023
SRR4895957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962016
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA1965800.0142.277732
TTCACGC1967150.0142.180071
CACGCAT615800.0141.90513
ACGCATG210300.0141.299264
CACGCAG642950.0141.145393
ACGCATA161350.0140.874634
ACGCATT178550.0140.75694
ACGCAGT199850.0140.663044
ACGCAAT155950.0140.633674
CACGCAA473600.0140.371053
CGCATGA53100.0139.197535
CGCATAG57100.0139.182945
ACGCAGG225450.0139.107224
ACGCAGA161400.0138.711234
CACGCAC254150.0138.009983
ACGCAAG158100.0137.774464
CGCAATG55150.0137.771355
CGCATTA45700.0137.094455
CGCAGTA58700.0137.089165
CGCAATA41500.0137.057255
Produced by FastQC (version 0.11.5)