FastQCFastQC Report
Sun 19 Mar 2023
SRR4895957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962016
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG167760.8550388987653516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC109590.5585581361212141No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC81910.4174787565442891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC79630.40585805620341525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65200.3323112553618319No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG50020.2549418557239085No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA37160.18939702836266373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC27640.14087550764112017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC25730.13114062270644072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA24950.12716511995824703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA24740.12609479229527182No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT24280.12375026503351653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC22930.11686958720010439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA20350.10371984734069448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20190.10290435959747525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT613950.0139.988461
GGTATCA617600.0139.298028
GTGGTAT620350.0138.541246
GTATCAA622000.0138.358939
TGGTATC624450.0137.793037
AGTGGTA624100.0137.731875
CAGTGGT626950.0137.105764
AGCAGTG628100.0136.731572
GCAGTGG635550.0135.196783
CAACGCA616050.028.50002910-14
TCAACGC617400.028.44003910-14
GTACATG613000.028.38108820-24
ATCAACG618550.028.3708710-14
TACATGG613500.028.32979220-24
AGAGTAC617500.028.26520315-19
CAGAGTA617800.028.24448815-19
AGTACAT616800.028.19456320-24
AACGCAG623400.028.15938410-14
CGCAGAG621750.028.13684315-19
GCAGAGT625000.027.89607415-19
Produced by FastQC (version 0.11.5)