FastQCFastQC Report
Sun 19 Mar 2023
SRR4895956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900608
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG211911.1149590025928544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT166390.8754566959625552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA147010.7734893255210964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC141250.7431832339967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC134590.7081418156716167No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT73530.38687619961612285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67690.35614919015388763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA57820.30421843957302086No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC43150.22703261272182376No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA38150.20072524160689634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA37710.19841019294878273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC36470.1918859649122807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA35630.1874663265649729No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC31830.16747272451762804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC28410.14947848267501768No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA24990.1314842408324073No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG23820.1253283159915143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGATGCAAATGTTTTGAAA23180.12196097248880358No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGCAGCCC23110.1215926692931946No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT22980.12090867764420647No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCAATAGATATAGTACCG21860.11501582651446275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGAGATTCCCCCAGTAG21430.11275339259857897No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGAAATCAACCGAGAT21350.11233247466074013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATTCCCCCAGTAGCGGC21170.11138540930060277No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20310.10686054146883522No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCACACCCGTCTATGTAG19840.10438764858403207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT685550.0140.97781
GGTATCA695050.0139.29348
GTATCAA697900.0138.703929
GTGGTAT701150.0138.040476
TGGTATC702850.0137.747567
CAGTGGT703050.0137.698144
AGCAGTG702100.0137.64442
AGTGGTA704250.0137.443055
GCAGTGG706350.0136.907973
TCAACGC694700.028.50283810-14
ATCAACG694250.028.48812710-14
AGAGTAC691300.028.47222115-19
GTACATG689800.028.41097320-24
CAGAGTA693000.028.40237415-19
CAACGCA698100.028.37433410-14
TACATGG691150.028.3388120-24
AGTACAT692400.028.2751720-24
GCAGAGT695750.028.25699615-19
CGCAGAG702350.028.11858215-19
AACGCAG708450.027.9496410-14
Produced by FastQC (version 0.11.5)