FastQCFastQC Report
Sun 19 Mar 2023
SRR4895955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2245061
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG380191.6934506456617437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC275021.2250001224911038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC204740.9119574033845852No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC112080.49922919689041856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAATCAACCGAGA78070.3477411081480637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG70670.3147798656695742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61220.27268746818015194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT56340.25095086503217506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT51930.23130774620377798No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACA50730.22596267985591484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT47580.21193188069277405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC47050.20957114305580116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACCGAGATTCCCCCAGTAG44940.2001727347274751No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGATTCCCCCAGTAGCGGC44700.19910372145790248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCGAACGGGGAGCAGCCC39780.17718894943166355No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAATCAACCGAGATTCCCC38290.17055215871640014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAGAAATCAACCGAGAT35750.15923843494675646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA32530.144895840246657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTAATGAGGCGAACC32350.14409408029447754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAACCGAGATTCCCCCAGTA31370.13972894277705594No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA30890.1375909162379107No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCATCTAAGTACCCCGAGGAA29100.12961785893568148No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC28990.1291278945204607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT28460.1267671568834878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC27280.1215111749747557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAACCGGGGGAACTGAAACA26100.11625519306602358No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA25400.11313723769643676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAGGAACACAGTAAACT25370.11300361103774018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGAGATTCCCCCAGTAGCGG24170.10765854468987703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTTCTTGCTGCAGCAACG22980.1023580205615794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCTAAGTACCCCGAGGAAA22630.10079904287678598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT783000.0140.158481
GGTATCA791050.0138.980338
GTATCAA798100.0137.797769
CAGTGGT798950.0137.552034
GTGGTAT802750.0136.8746
AGTGGTA803550.0136.755635
AGCAGTG802250.0136.696642
TGGTATC805350.0136.485757
GCAGTGG807900.0135.954593
CAACGCA788550.028.5710510-14
TCAACGC790700.028.48425710-14
ATCAACG792050.028.41934610-14
GTACATG787950.028.37281820-24
AGAGTAC791100.028.36648215-19
TACATGG788250.028.35653920-24
CAGAGTA792500.028.32545515-19
AGTACAT791700.028.22751220-24
CGCAGAG797000.028.18901415-19
AACGCAG803950.028.01838710-14
GCAGAGT801050.027.98537415-19
Produced by FastQC (version 0.11.5)