FastQCFastQC Report
Sun 19 Mar 2023
SRR4895954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059545
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA3082300.0141.497091
GAACAAT1017700.0140.849663
GGAACAA3106450.0140.397062
GAACAAG1059350.0139.436373
AACAATG349950.0135.097084
GAACAAC380200.0135.015293
AACAAGT350300.0133.598834
GAACAAA720500.0130.116623
ACAATAG102750.0130.065285
ACAATGG122450.0130.036825
ACAAGTG117600.0129.338875
AACAAGG389650.0129.033574
ACAATCG38200.0128.184455
AACAATA291850.0127.629664
ACAAGTA108300.0127.546115
ACAATTG122700.0127.448285
ACAATGT127950.0125.5055165
ACAATGA97350.0124.8332755
AACAATT351050.0123.851944
AACAACG120450.0123.4381564
Produced by FastQC (version 0.11.5)