FastQCFastQC Report
Sun 19 Mar 2023
SRR4895954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059545
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG324131.0594058920525764No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC206200.6739564216247841No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC123820.40470069896013955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92470.3022344825782919No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG79100.2585351743478197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC51170.1672470906621736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC45720.14943398446501033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA815450.0138.63748
GTGGTAT816000.0138.455726
AAGCAGT818600.0138.052541
TGGTATC821600.0137.582127
GTATCAA829300.0136.330739
CAGTGGT834350.0135.514194
AGCAGTG834650.0135.346052
AGTGGTA835300.0135.273885
GCAGTGG843050.0134.091483
TCAACGC811400.028.49980710-14
CAACGCA812000.028.47697810-14
ATCAACG817300.028.26588210-14
GTACATG812750.028.23268120-24
TACATGG814250.028.1718320-24
AGAGTAC820650.028.0808615-19
AGTACAT818150.028.04633720-24
CAGAGTA823150.028.00257315-19
CGCAGAG824750.027.9831715-19
AACGCAG833500.027.75623910-14
GCAGAGT833250.027.65450915-19
Produced by FastQC (version 0.11.5)