FastQCFastQC Report
Sun 19 Mar 2023
SRR4895953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838694
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG842650.0141.881031
GAAGAGA847850.0141.010862
AAGAGAT272250.0139.258883
AAGAGAG277950.0137.916093
AAGAGAC102600.0135.193653
AAGAGAA221500.0132.903723
AGAGACC36500.0126.934984
AGAGATC33300.0126.7179954
AGAGACT23750.0126.351174
AGAGATG100950.0126.317884
AGAGAGT88900.0126.201294
AGAGATA84750.0125.904984
AGAGACG30250.0124.649674
AGAGAGC40500.0123.198054
GAGACGT9550.0123.151985
AGAGATT84200.0121.9867554
AGAGAAT77800.0121.5771944
GAGATCA7450.0120.552185
AGAGAAG80950.0120.016184
GAGATAG27300.0119.842495
Produced by FastQC (version 0.11.5)