FastQCFastQC Report
Sun 19 Mar 2023
SRR4895953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838694
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG66480.7926609705089103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC44640.5322561029410011No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA36880.43973129651577336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32780.390845767347805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC25350.3022556498556088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC20280.2418045198844871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA18500.2205810462457106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT18340.21867331827818012No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTTTCTTGCTGCAGCAACG15580.1857650108382795No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA14160.1688339251264466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCTAAGCTGCAGCAAGAG12130.14462962653840375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCTAAGCTGCAGCAAGA10520.1254331138651284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC10050.11982916296050763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT9660.11517907603965213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC9410.11219825109038577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212400.0139.897558
AAGCAGT212600.0139.782611
GTGGTAT213850.0138.982656
GTATCAA214400.0138.65979
CAGTGGT215350.0138.048024
TGGTATC215700.0137.757267
AGTGGTA216350.0137.31015
AGCAGTG216800.0137.008222
GCAGTGG217650.0136.473163
CGTTGCG200.00628574928.79984555-59
CAACGCA212800.028.51563510-14
AGAGTAC211900.028.4668615-19
ATCAACG213050.028.45514110-14
TCAACGC213500.028.42888810-14
TACATGG210850.028.41739720-24
GTACATG211500.028.3777220-24
CAGAGTA213000.028.31984715-19
AGTACAT212600.028.2308920-24
CGCAGAG214850.028.19663615-19
GCAGAGT214200.028.12085515-19
Produced by FastQC (version 0.11.5)